Structure and Function of Biomolecules - Midterm Exam II Solved | BIS 102, Exams of Biology

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Copyright UC Regents Davis campus, 2005 - 15. All rights reserved. May not be re-distributed without prior written consent of course instructor. . lof 3 BIS 102 Name Ken Fall, 2015 Last ~ First K. Tilt Second Midterm Score (100): Equations: PH ~ pK, + log {[bJ/laJ} (K.) (Ky) = 1 x 10-4 Ky =x (y-x) Ka> x2/(y-x) pH= (pKa, +pKy,V2 F=(q'q’)/er? = AG=AH- TAS pK,’s of amino acid side chains: _D (3.9), F (4.2), H 6.0), C (8.3), ¥ (10.1). K (10.5), R (12.5) PK, of amino acid a-COOH (2.1); pK, of amino acid a-NH3* (9.6) pK, of oligopeptide N-terminus (7.4); pKa of oligopeptide C-terminus (3.6) pK, of NHs* (9.25) 1. Amino acid analysis is done on protein “7”. ‘Lhe following results are obtained: Amino Acid Number of Residues Amino Acid Number of Residnes in ; in Protein “7” _ Protein “Z” A 13 B 14 ~ ty G 12 Ww 6 Pp 5 H 9 3 Zz 240 24 c 23 W-H — -21. 307d K [44 B-W 12.47 V 24 __ R 2 +2 8 12 L 23 T 14 Y 17 H-H - -2.927 E 13 L NU! 9 +4 N-jem. =O c- term, =—| (30 pts.) a) Calculate the net charge, to the nearest 0.01, of protein “Z” at pH _9.4. Show all calculations. Put your final answer here: ~24-\% _. You may use the back of this page. K } C0,4264 Yl) =U12 ahs ie : toc 44 = 1D.) , 2 Bl + bog, Net chomge = -~29,1LY2 ~ OTE log & Oo _ oa = ib Blas = by ha O17 _ 4 yyyg = 44. ae S = “ (é. '663)-ON(17) = Cz2.¢28) (5 pts.) _b) Does protein “Z” have quaternary structure? If it does, how would you experimentally confirm this, using the techniques that we have discussed in this portion of the course? If there is more than one technique that could be used, then list them all. We Lo net knoe frm He aloe in Eecmation . we etd uses t) Reva en Stel luv ghey ey 2) Sande yeagext (TDNO), a J 0 se , 5: wy he (aKa only works YE the eudsaaits “pene Ayfferedt N-t+ermin -