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Post tutorial assignment, Assignments of Biology

2023-2024 year biology 1A03 assignment

Typology: Assignments

2023/2024

Uploaded on 09/17/2024

qiu-kelly
qiu-kelly 🇨🇦

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Post-lab 1 assignment
Data collected:
1. Standard curve of trail 1
y = 0.0611x + 0.038
R² = 0.9951
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
0 5 10 15 20 25
absorbance at 665 nm
methylene blue molarity (uM)
Absobance Trail 1
pf3

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Post-lab 1 assignment

Data collected:

1. Standard curve of trail 1

y = 0.0611x + 0. R² = 0. 0

absorbance at 665 nm

methylene blue molarity (uM)

Absobance Trail 1

Standard curve of trail 2

2. My R square values for trail 1 is 0.9951 and for trail 2 is 0.9938 of the methylene blue

standard curve. The differences between two values might cause by human error,

such as incorrect of using micropipette and not enough time for mixing the DI water

and methylene blue. It might also cause by non-human error, such as the incorrect

reading of the spectrophotometer, or smaller amount of liquid stuck on the side of

beaker which caused volume not exactly accurate.

y = 0.0695x + 0. R² = 0. 0

Absorbance at 665 nm

methylene blue molarity (uM)

Absorbance Trail 2