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MICROBIOLOGY MIDTERM 1 EXAM WITH CORRECT AND VERIFIED ANSWERS 2025 - UNIVERSITY OF CALIFORNIA, DAVIS (UC DAVIS)
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Pathogenic - correct answers Cause infection/disease, food spoilage Eukaryotic vs. Prokaryotic - correct answers Prokaryotic cells: Lack membrane-bound organelles Lack a nuclear membrane Generally smaller in size compared to eukaryotes Peptidoglycan - correct answers Makes up bacterial cell wall Bacillus - correct answers rod-shaped Coccus - correct answers Spherical Spiral - correct answers corkscrew-shaped Archaea - correct answers Single-celled organisms, have a cell wall (no peptidoglycan)
Structure and function are very similar to bacteria Non-pathogenic to humans Nutrition: environmental nutrients, photosynthesis/carbon fixation Methanogens - correct answers Archaea produce methane as a waste product Extreme halophiles - correct answers Archaea live in extremely salty environments Extreme thermophiles - correct answers Archaea live in extremely hot environments Protozoa - correct answers Eukaryotic Unicellular organisms, no cell wall Exist in a variety of shapes and sizes Move using pseudopods (extensions of the cytoplasm), flagella, or cilia Can live as freely as self-sufficient organisms, but also as parasites
Zacharias Jansen - correct answers Discovered/invented compound light microscope Robert Hooke - correct answers first person to describe microorganisms Cell Theory - correct answers all living things are composed of cells Leeuwenhoek - correct answers 1600s; magnifying lens and microscope; looked at bacteria, red blood cells, and yeast theory of spontaneous generation - correct answers states that life can spontaneously emerge from nonlife Louis Pasteur - correct answers showed that microorganisms are present in air and can contaminate sterile solutions, but that the air itself does not generate those microbes fermentation reactions - correct answers Yeasts can convert sugars to alcohol to make wine and beer in the absence of oxygen
When exposed to air, alcohol is converted to acetic acid (vinegar) by bacteria, making it sour pasteurization - correct answers partial sterilization of foods at a temperature that destroys harmful microorganisms without major changes in the chemistry of the food germ theory of disease - correct answers idea that infectious diseases are caused by microorganisms, or germs Semmelweis - correct answers disinfecting hands reduced disease transfer Lister - correct answers started applying phenol to surgical wounds to kill bacteria it worked so well that other surgeons adopted it Heat-sterilized instruments Koch's Postulates - correct answers 1) Pathogen must be present in all disease cases
Differential Stains - correct answers Used to differentiate organisms into two different categories Don't stain all cells the same colour eg. Gram stain Gram positive - correct answers cells are purple d/t crystal violet Gram negative - correct answers cells are pink because of the counterstain (safranin) Theory of RNA World - correct answers the idea of self-replicating RNAs carrying out all of the processes necessary for their replication Montmorillonite clay - correct answers Lipid vesicles spontaneously form, very similar to cells Can also cause spontaneous formation of RNA oligonucleotides stromatolites - correct answers layered rocks that form when certain prokaryotes bind thin films of sediment together
cyanobacteria - correct answers Photosynthetic, oxygen-producing bacteria (formerly known as blue-green algae).
Proteobacteria - correct answers Sulfur-oxidizing bacteria Methanotrophs and Methylotrophs, - correct answers use methane (CH4) and other one-carbon compounds as electron donors for metabolic processes Pseudomonads - correct answers each species can use a variety of carbon sources for metabolic processes (between 20-100 depending) Vibrio sp. and Photobacterium sp. - correct answers curved rods, mostly aquatic and found in either freshwater or marine habitats, and some are bioluminescent luciferase - correct answers enzyme involved in bioluminescence, produced by Photobacterium magnetosomes - correct answers Contain crystals of magnetite or greigite, allowing cells to respond to magnetic fields. Sheathed Proteobacteria - correct answers filamentous bacteria special life cycle, involving the release of swarmer cells from the sheath to facilitate their dispersal
GC - correct answers Guanine-Cytosine content Non-sporulating, low GC - correct answers Staphylococcus sp. Micrococcus sp. Sarcina sp. Streptococcus sp. Lactobacilli sp. Listeria sp. Acetic acid bacteria - correct answers perform incomplete oxidation of alcohols and sugars, leading to the production of organic acids.
Lactobacilli - correct answers fermentative, acid-resistant bacteria found commonly in dairy products Listeria sp. - correct answers fermentative coccobacilli Endospore-forming, low GC - correct answers Bacillus sp. Clostridium sp. Bacillus sp. - correct answers produce antibiotics are toxic to many insect larvae some are extremely pathogenic to humans Clostridium sp. - correct answers responsible for several human diseases including tetanus, botulism, and gas gangrene Actinobacteria, High GC - correct answers Corynebacteria sp. Mycobacterium sp. Streptomyces sp.
Acid-fast - correct answers Acid-fast retain the red stain, Non-acid-fast organisms do not, and appear blue Corynebacteria sp. - correct answers v-shaped caused by a snapping movement occuring after cell division Mycobacterium sp. - correct answers contain special lipids called mycolic acids that cause them to stain acid-fast Streptomyces sp. - correct answers extremely diverse and are known for being very filamentous laments form a mycelium, which is characteristic of many fungi Cyanobacteria Definition - correct answers photosynthetic: they have one chlorophyll form (chlorophyll a)
Elementary bodies - correct answers found in Chlamydia sp. small, dense cells that are resistant to the environment Used for dispersal of the pathogen Reticulate bodies - correct answers Found in Chlamydia sp. divides readily and is the vegetative form once inside the host cell Planctomyces sp. - correct answers ONLY PROKARYOTES SHOWN TO HAVE COMPARTMENTALIZATION Planctomyces - correct answers considered to be stalked bacteria --> the stalks are made of protein Verrucomicrobia - correct answers Named for their "warty" appearance, members of this species exhibit prostheca prostheca - correct answers cytoplasmic appendage of Verrucomicrobia sp Flavobacteria genus - correct answers Bacteroides sp. Flavobacterium sp.
Flavobacteria - correct answers extremely variable and can be found in many different habitats, and some members are psycrophilic or psychrotolerant psycrophilic - correct answers bacteria that grow in cooler temperatures psychrotolerant - correct answers can grow at cold temperatures Bacteroides sp. - correct answers present in human feces (10^10 cells per gram), and are known for synthesizing sphingolipids Cytophaga Group - correct answers Rods with pointed ends
Deinococcus radiodurans - correct answers Extremely resistant to radiation (can withstand 10x more radiation than a human)
Euryarchaeota - correct answers Some members of this phylum extremely halophilic Presence of salt is required for them to survive bacteriorhodopsin - correct answers Pigmented protein found in the plasma membrane of a salt-loving euryarchaeote