Docsity
Docsity

Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity


Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan


Guidelines and tips
Guidelines and tips

Malaria Parasites: A Laboratory Exercise on Plasmodium Morphology and Diagnosis, Lab Reports of Parasitology

This laboratory exercise focuses on the identification and diagnosis of malaria parasites, specifically the genus plasmodium. Students will learn to identify the morphology of different plasmodium stages through microscopic examination of blood smears. The exercise includes guided questions and instructions for drawing or photographing parasites, labeling their structures, and identifying potential species. It also explores the life cycle of plasmodium, its hosts, and methods for diagnosis and treatment in various species, including chickens. The exercise provides a practical approach to understanding the morphology and life cycle of malaria parasites, essential for students in biology, parasitology, and related fields.

Typology: Lab Reports

2023/2024

Uploaded on 09/05/2024

gwen-alegre
gwen-alegre 🇵🇭

1 document

1 / 6

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
Exercise No. 15: Malaria Parasites
Introduction
The Genus Plasmodium contains the malarial organisms of man, other mammals, and
vertebrates. In terms of the life cycle, the asexual process schizogony occurs in the erythrocytes
and endothelial cells of the inner organs of the vertebrate host, depending on
the Plasmodium species. The sexual phase of the cycle occurs in the blood-sucking insects,
which for mammalian forms are anopheline mosquitoes while culicine mosquitoes are for the
avian forms (Taylor et al., 2016). In this exercise, you will learn to diagnose malaria based on the
parasite morphology from a blood smear.
Learning Outcomes
1. Identify the morphology of different stages of Plasmodium.
2. Formulate a process for Plasmodium diagnosis.
Materials
· Prepared slides of Plasmodium species
· Microscope with an oil-immersion objective lens
Procedure
A. Examination of the blood smear
1. Focus on the blood smear and study the location of the parasite in relation to the blood cells
(see Figure 1A-C).
2. Examine the parasite by taking note of their structures.
pf3
pf4
pf5

Partial preview of the text

Download Malaria Parasites: A Laboratory Exercise on Plasmodium Morphology and Diagnosis and more Lab Reports Parasitology in PDF only on Docsity!

Exercise No. 15: Malaria Parasites Introduction The Genus Plasmodium contains the malarial organisms of man, other mammals, and vertebrates. In terms of the life cycle, the asexual process schizogony occurs in the erythrocytes and endothelial cells of the inner organs of the vertebrate host, depending on the Plasmodium species. The sexual phase of the cycle occurs in the blood-sucking insects, which for mammalian forms are anopheline mosquitoes while culicine mosquitoes are for the avian forms (Taylor et al., 2016). In this exercise, you will learn to diagnose malaria based on the parasite morphology from a blood smear. Learning Outcomes

  1. Identify the morphology of different stages of Plasmodium.
  2. Formulate a process for Plasmodium diagnosis. Materials · Prepared slides of Plasmodium species · Microscope with an oil-immersion objective lens Procedure A. Examination of the blood smear
  3. Focus on the blood smear and study the location of the parasite in relation to the blood cells (see Figure 1A-C).
  4. Examine the parasite by taking note of their structures.

Figure 1A-C. Blood parasites. B. Drawing or photograph

  1. Draw or photograph the whole microscope field with the parasites.
  2. Focus on one parasite and draw the structures.
  3. Indicate the estimated length and width.
  4. Label the structures observed.
  5. Identify the possible species.

Guide questions and instructions on how to prepare and submit the laboratory

  1. Illustrate or draw the different stages of Plasmodium affecting primates and avians? ( points)
  1. What are the specific intermediate and definitive hosts of Plasmodium knowlesi? (5 points) ➢ The intermediate host of Plasmodium knowlesi is Macaca fascicularis. ➢ The definitive host of Plasmodium knowlesi is a mosquito.
  2. How do you diagnose and treat Plasmodium species affecting chicken? (5 points) ➢ The infections that is caused by Plasmodium spp causes clinical and rarely fatal disease in chicken and other fowl. Nonspecific clinical signs result from anemia and diagnosis can be made using tissue cytology, histopathology, blood smears and PCR. Limiting the mosquito vectors to help control the disease since there’s no treatment yet that is approved by poultry.
  3. How does Plasmodium differ from Babesia in terms of morphology and life cycle? ( points) ➢ Plasmodium is referred to as digenetic parasites since they are obligate parasites of insects such as mosquitoes and vertebrates. Two different hosts are require in order to complete their life cycle while in vertebrates they multiply within the liver cells and red cells where they obtain nourishment and damage the cells which causes the disease. While the Babesia is a parasite that is transmitted to mammalian host by arachnid (tick) vectors and they do not produce spores, flagella, cilia or pseudopodia but they move by body flexion or gliding. In mammals, they do not undergo exoerythrocytic schizogony,