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Microbiology Sample Collection and Diagnostic Tests, Quizzes of Microbiology

The different types of samples that can be collected for microbiological analysis, such as blood, urine, feces, abscesses and wounds, throat/nasal swabs, and genital samples. It also explains various diagnostic tests including selective and differential media, minimum inhibitory concentration, disk diffusion assay, etest, serology, antigens, antibodies, agglutination, hemagglutination, serotyping, immunofluorescence, enzyme immunosorbent assays, direct and indirect immunoassays, quantitative real-time pcr, and reverse-transcription pcr reaction.

Typology: Quizzes

2015/2016

Uploaded on 11/16/2016

nguyenbt96
nguyenbt96 🇺🇸

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TERM 1
Blood
DEFINITION 1
bacteremia, viremia, antibodies
TERM 2
Urine
DEFINITION 2
bacteriuria
TERM 3
Feces
DEFINITION 3
look for intestinal pathogens
TERM 4
Abscesses and wounds
DEFINITION 4
swab or needle aspiration
TERM 5
Throat/nasal swabs
DEFINITION 5
soft cotton swabs to collect samples to test for bacteria or
viruses
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Blood

bacteremia, viremia, antibodies

TERM 2

Urine

DEFINITION 2

bacteriuria

TERM 3

Feces

DEFINITION 3

look for intestinal pathogens

TERM 4

Abscesses and wounds

DEFINITION 4

swab or needle aspiration

TERM 5

Throat/nasal swabs

DEFINITION 5

soft cotton swabs to collect samples to test for bacteria or

viruses

Genital

samples

to get specimens to test for sexually transmitted pathogens

TERM 7

Selective medium

DEFINITION 7

has ingredients that inhibit some microbes

TERM 8

Differential medium

DEFINITION 8

look for biochemical pathways (color change)

TERM 9

Min. inhibitory concentration

DEFINITION 9

lowest [] that inhibits growth of microbes

TERM 10

Disk diffusion assay (Kirby-Bauer test)

DEFINITION 10

1. Pure culture2. Smear onto agar3. Add paper disk4. Growth

for 24 hour

antibody titer

[ ]

TERM 17

Mantoux skin test

DEFINITION 17

common for testing of previous exposure or current infection

with M. TB

TERM 18

Agglutination

DEFINITION 18

clumping

TERM 19

Hemagglutination

DEFINITION 19

use for blood type

TERM 20

Serotyping

DEFINITION 20

use antibodies to discriminate different type of pathogens

Direct immunofluorescence

take antibody that's purified

TERM 22

Indirect immunofluorescence

DEFINITION 22

2 layers of antibodies (primary and secondary)-increases the

sensitivity of detection

TERM 23

Enzyme Immunosorbent Assays (EIA)

DEFINITION 23

ELISAtake an antibody and stick an enzyme on itcolorless

substrate ---enzyme-> colored product

TERM 24

Direct imunoassay

DEFINITION 24

use immobilized antibody to test for an antigen from the

patient1. add antibody2. wash3. add patient sample4. wash5.

add enzyme

TERM 25

Quantitative Real-Time PCR (qPCR)

DEFINITION 25

Requires PCR thermocycler-fluorescence increases as double

stranded DNA product is made