CHEM 330 FINAL EXAM
What amino acid contains a hydroxyl group in its side chain? ✔✔serine
What makes proline a distinct amino acid in proteins? ✔✔contains a cyclic structure; has the most
strongly basic side chain group; and has a secondary amino group at the alpha carbon
With respect to stereochemistry, naturally occurring proteins contain what? ✔✔L-amino acids
What is the purpose of treating a proteins with 2-mercaptoethanol? ✔✔to cleave disulfide bonds
present in the protein
The amino acid composition often cannot be determined precisely after acidic hydrolysis because...?
✔✔side chains asparagine and glutamine are hydrolyzed
The Edman degradation technique is used to....? ✔✔sequence a peptide from the N-terminal end to the
C-terminal end
What method separates peptides based on their net electrical charge? ✔✔affinity chromatography
What methods separate peptides based on their size? ✔✔SDS-PAGE Electrophoresis and
ultracentrifugation
What are mirror images? ✔✔enantiomers
What are diastereomers? ✔✔stereoisomers that are not enantiomers
What is a stereoisomer with a different configuration at the anomeric carbon (2nd to last carbon)?
✔✔anomer
The cyclization of glucose forms what? ✔✔an additional chiral center in the molecule
