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2018-2019, fall semester – Biophysics Practice Questions for Nucleic Acids Lecture
Y. Adıgüzel
1. Which one below is not among the functions of nucleic acids in the cells?
a) they are used as the building blocks of DNA for information storage and transmission b) they are used as the building blocks of RNA for information transmission and cellular functions c) they constitute part of the coenzymes d) they take role in the energy storage e) they can act as second messengers f) all are among their functions
2. In which type of the molecules the hydrogen bonding between the nucleotide bases are observed to be more extensive in general, DNA or RNA?
a) DNA b) RNA c) it is the same in both
3. There are 3 hydrogen (H-)bonds between the GC base pairs and 2 H-bonds between the AT base pairs. Would the melting temperature of DNA increase or decrease with increasing GC content?
a) increase b) decrease c) does not change
(ds: double-stranded, ss: single-stranded)
4. The above curve presents the melting curves of DNA molecules with varying GC contents. Which one of the curves among curve-a and curve-b belongs to that with higher GC content?
a) curve-a b) curve-b c) none, both have the same GC content
5. ____________ of a DNA duplex increases due to the electrostatic shielding of its anionic phosphate groups when the cationic concentration of the medium increases. What is the most appropriate expression among those given below in the blank region of the sentence?
a) melting temperature (Tm) b) base pairing c) complementarity of base pairs
6. Which one below does not / which ones below do not contain nucleic acids as the building blocks?
a) only DNA b) only RNA c) DNA and RNA d) lipids e) proteins f) lipids and proteins
7. Which one below is not used to study DNA?
a) X-ray crystallography b) NMR c) DNA purification d) PCR e) electrophoresis f) none, all used
8. Which form of DNA given below is more common in nature?
a) A b) B c) Z d) none, all are equally common
9. Which part is likely to be different between the nucleic acids (na) of DNA?
a) sugar groups b) phosphate groups c) nitrogenous bases d) none, all are the same in na
10. What is the name of the parts of DNA that are responsible for the protein synthesis?
a) nucleic acid b) RNA c) gene d) protein e) none of those
11. All genes contain essentially the same number of nucleic acids. Is this true or false?
a) true b) false c) none
12. Which one below is not about the organization in DNA?
a) information in DNA is organized in the form of nucleic acid sequences b) information in DNA is organized in the form of genes and their regulatory regions c) structure of DNA is organized through its interactions within itself and with proteins d) none, all of those are about the organization in DNA
13. Which property below is likely to be not affecting or least affecting the DNA’s electrophoretic mobility?
a) length of the DNA b) strength of the applied voltage c) conductivity of the running buffer c) number of copies of the same length of DNA d) none of those, all are affecting to the same extent
14. Which one below can result in two cells with the same genome to act differently?
a) different number of proteins are expressed by the genes b) different proteins are expressed by the genes c) they can have different extracellular pH d) they can have different intracellular pH e) all of those above
15. Which one below is the right hierarchy until the chromosome?
a)DNA double helixDNA wrapped around proteinschromatin fiberloop foldinghelical folding b)DNA wrapped around proteinschromatin fiberloop foldinghelical foldingDNA double helix c)chromatin fiberloop foldinghelical foldingDNA double helixDNA wrapped around proteins d)loop foldinghelical foldingDNA double helixDNA wrapped around proteinschromatin fiber e)helical foldingDNA double helixDNA wrapped around proteinschromatin fiberloop folding
