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Cell Origins & Evolution: Prokaryotic, Eukaryotic Lives & Endosymbiotic Theory, Schemes and Mind Maps of Biochemistry

The origin and evolution of cells, focusing on the similarities between prokaryotic and eukaryotic lives, the endosymbiotic theory, and the role of experimental models in cell biology. It discusses the concept of the last universal common ancestor (LUCA), the differences between prokaryotic and eukaryotic cells, and the endosymbiotic origin of mitochondria and chloroplasts.

Typology: Schemes and Mind Maps

2020/2021

Uploaded on 10/22/2022

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Same basic molecular mechanisms governs
prokaryotic and eukaryotic lives, indicating that all
present-day cells are descended from asingle first
ancestor
1. The origin and evolution of cells (Cooper’s book)
3. Prokaryotes
4. Eukaryotic cells
2. The endosymbiotic theory and cell specialization
3. Experimental models in cell biology
Prokaryotic cells
Cells
Eukaryotic cells
UNIT 1
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pf5
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Same basic molecular mechanisms governs prokaryotic and eukaryotic lives, indicating that all present-day cells are descended from a single first ancestor

  1. The origin and evolution of cells (Cooper’s book)
    1. Prokaryotes
    2. Eukaryotic cells
  2. The endosymbiotic theory and cell specialization
  3. Experimental models in cell biology

Prokaryotic cells

Cells

Eukaryotic cells

UNIT 1

3. Prokaryotes

LUCA last universal common ancestor Prokaryotes Archaea

Bacteria

Eukaryotes

All organisms existing today come from the “LUCA” cell. This cell is the beginning of the tree of life, then, we now classified all organisms in three main domains: The Bacteria, the Archaea (both procariotic cells) and the eucaryote domains. Bacteria and archaea diverge early in the evolution and, from archaea, evolve the eukaryotic domain.

Cells

3. Eukaryotic cells

a) Nucleus b) Mitochondria c) Golgi Apparatus d) Vacuole e) Chloroplast f) Lysosome g) Cell Membrane h) Endoplasmic Reticulum i) Ribosome j) Cell Wall

Cytoskeletom The structural framework of the cell: Maintains the internal organization of the cell. It is made of protein filaments extending throughout the cytoplasm

Cell shape Cell movement Intracellular transport Organelle positioning

Structures for chromosomes movement

Membrane-enclosed subcellular organelles

  • Nucleus,
  • Endoplasmic reticulum
  • Golgy apparatus 4. Eukaryotic cells

Invaginations of the plasma membrane

Many cell structures are formed by internal membranes, so the acquisition of these internal membranes allowed the cell to isolate some regions to perform certain functions independently from the rest of the cell

Some organelles evolve from invaginations of the plasma membrane:

The genome of eukaryotes arose from a fusion of archaeal and bacterial gemones

The initial endosymbiotic relationship of bacterium living inside an archaeum organism gave rise not only to mitochondria (or chloroplast) but also to the genome of eukaryotic cells containing genes derived from both procaryotic ancestors.

1.2. The endosymbiotic theory

As a result of all this processes the genome of eucaryotic cells is a mix of bacteria's and archaeal related genes. Curiously most eukaryotic genes implicated in formational processes, such as DNA replication, transcription and protein synthesis are related to arqueal genes. By contrast, most eucaryotic genes related to basic cell operational processes such as glycolysis and amino acid biosynthesis derived from bacteria genes. So, the endosymbiotic theory not only explains the origin of mitochondria and chloroplast but also the origin of the eucaryotic genome.

1.2. Cell specialization, The development of multicellular organisms

yeast

The ciliated protozoan Paramecium Amoeba Dictyostelium

Understanding the mechanisms that control the growth and differentiation of such a complex array of specialised cells starting from single fertilized egg, is one of de mayor challenges facing contemporary cell and molecular biology

1.3 Experimental models in cell biology

2 Yeasts , the simplest model for eukaryotic cells (Saccharomyces cerevisiae)

  • More complex, but still grow easily under certain laboratory conditions
  • divides every 2 hours
  • Genome: 12 million base pair – 6000 genes
  • It contains nuclear membrane and subcellular organelles
  • The DNA is organized in 16 linear chromosomes

From this model we learnt how an eucaryotic cell Works:

  • DNA replication, RNA processing, protein sorting and the regulation of cell división.

3 Caenorhabditis elegans (c.elegans) A multicellular nematode (a worm)

  • It grows easily and can be genetically modified
  • divides every 2 hours
  • Genome: 100 million base pair – 19.000 genes
  • Adult worms have 959 somatic cells and 1000-2000 germ cells
  • It has been extensively studied under the microscope.
  • It is very interesting model for animal development and cell differentiation

4. Drosophila melanogaster - Genome: 180 millions base pair - 14,000 genes - Reproduction cycle: two weeks

Very useful organism for genetic experiments, the relationship between genes and genomes has been extensively studied in this organism

1.3 Experimental models in cell biology

5. The Zebrafish (26000 genes)

  • They can be easily maintained in a laboratory
  • They reproduce quite rapidly (every three to four months) -The embrios develop outside of the mother and are transparent, so, first steps in development can be easily observed
  • Many mutations have been already identified.

A very interesting model to study the cardiac system.

PROBLEM

An adult human is composed of about 10 13 cells, all of which are derived by cell division from a single fertilized egg.

  1. Assuming that all cells continue to divide (as for example, bacteria cells) how many generations of cell divisions would be required to produce 10^13 cells?

2.Human cells in culture divide about once per day. Assuming that all cells continue to divide at this rate during development , how long would it take to generate an adult organism?

3.Do you think, that adult humans take longer to develop than these calculation might suggest?