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lof4 Biological Sciences 102 Name Keay Winter, 2010 TuTh Last, } First M. Etzler, K. Hilt Final Exam Score (100): Equations: pH = pK, +log {{bYfal} Ky=x'y-x) Kp=x7/(y-x) PH=(pKg,+pKg,V2 (KaX(Ky) = 1 x 10 F-@q'q@)/er AG=AH-TAS Vo = {Vuax [ST/ {Km + [S}3 Amino acid pK,’s; o-carboxyl group (2.1), a-amino group (9.6) side chains: D(3.9) E(4.2) H(60}) C(83) Y(10.1) K(05) R(12.5) Oligopeptide pK,’s: C-terminal carboxyl group (3.6), N-terminal amino group (7.4) 1. (10 pts.} The scheme shown below represents the oxygen equilibria between the protonated and unprotonated forms of an animal hemoglobin. (pKa: = 8.00; pKa2 = 6.24). HHgb + O2 + HHgbO. pKa, i pKa, I Hgb + O2 —> HgbO2 + + H* H* Calculate the number of moles of H* taken up by the hemoglobin per mole of O, released at pH 7.4. Put your answer here: 90-724 moles of H’. To receive credit, show all of your work on the back of this page. 2. (10 pts.) An enzyme catalyzed reaction was carried out in a solution containing 0.10 M Tris buffer. The pH of the reaction mixture at the start was 8.5. As a result of the reaction, 0.033 mole/liter of H' was produced. What was the final pH of the reaction mixture? The pK, of Tris is 8.1. Put your answer here: final pH= 7 -7_. To receive credit, show all of your work on the back of this or another page. (5 pts.) In question #2 above, the assay volume of the enzyme catalyzed reaction was 3.00 mi. The reaction described in question #2 ran for 5 minutes under Vmax conditions. Assume that the reaction catalyzed was S* 2 P +H". How many LU.’s of enzyme must have been in the assay? Show your calculations and reasoning in the space below. (2: 033 mek WY (o. 203 2\ = 4.9 47 mt He & m4 Dm HEL pce, = 44. R *% yo® rh A fn, md [men = 44% = G9. xu.) 
