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Questions And Answers
This DNA amplification method amplifies the nucleic acid used as a probe? - correct answer ✅Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - correct answer ✅DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - correct answer ✅Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - correct answer ✅Better specificity How does Ligase Chain Reaction differ from Polymerase Chain Reaction? - correct answer ✅This method uses a thermostable ligase to join to probes which are then amplified by standard PCR cycling. Why was Ligase Chain Reaction originally developed? - correct answer ✅To detect point mutation.
Questions And Answers
What do positive controls ensure during PCR testing? - correct answer ✅That the enzyme is active, the buffer is optimal, the primers are priming the correct sequence, and that the thermal cycler is working properly. What is a reagent blank control in PCR? - correct answer ✅A negative control that lacks DNA to ensure that the reaction mix is not contaminated with DNA template or amplified products from a previous run. What is a negative template control in PCR? - correct answer ✅A negative control that contains DNA that lacks this target DNA sequence. This ensures that the primers are not annealing to unintended DNA sequences. What is the purpose of the amplification control in PCR? - correct answer ✅To distinguish between a true negative for the sample and an amplification failure (false negative). What is the major source of contamination in PCR testing? - correct answer ✅Amplicons from previous runs.
Questions And Answers
What are three common molecular tests that involve the amplification of probe sequences? - correct answer ✅Ligase Chain Reaction (LCR), Strand Displacement Amplification (SDA), and Q-beta Replicase. What is isothermal amplification? - correct answer ✅A process that uses one temperature for amplification after the initial denaturation. The major amplification products in Strand Displacement Amplification (SDA) is? - correct answer ✅Probes/Primers Strand Displacement Amplification (SDA) was first widely used to detect which organism? - correct answer ✅Mycobacterium tuberculosis Where does Q-beta Replicase derives its name? - correct answer ✅From the major enzyme that is used to amplify the probe sequence (Q-beta replicase)
Questions And Answers
What is Q-beta Replicase? - correct answer ✅A RNA-dependent RNA polymerase Why are signal amplification tests better for quantifying the amount of target sequence in a sample? - correct answer ✅Because the number of target sequences are not amplified. What are three signal amplification techniques? - correct answer ✅Branched DNA Amplification (bDNA), Cleavage- Based Amplification, and Cycling Probe. What is the target nucleic acid for bDNA amplification? - correct answer ✅DNA or RNA Does Strand Displacement Amplification (SDA) require a thermocycler? - correct answer ✅No
Questions And Answers
In this amplification method, target sequences are detected using a synthetic probe consisting of sequences of DNA-RNA-DNA? - correct answer ✅Cycling Probe RNase H cleaves the RNA from the middle of the probe in what amplification technique? - correct answer ✅Cycling Probe How is the reporter dye and quencher dye separated in the Cycling Probe test? - correct answer ✅When the probe is digested. The Hybrid Capture Assay resembles with chemistry/EIA technique?
correct answer ✅Sandwich Assay Primer dimers result from? - correct answer ✅3' complementarity in the sequence primer What control is ran to detect contamination in a PCR test? - correct answer ✅Reagent Blank
Questions And Answers
Nonspecific extra PCR products can result from? - correct answer ✅Mispriming How many copies of a target is made after 20 cycles of PCR? - correct answer ✅2^ What are the three basic steps in a PCR cycle? - correct answer ✅Denaturation, annealing, and extension Real-time PCR is considered to be a Qualitative or Quantitative test? - correct answer ✅Quantitative
